Microscope

=**Different type of Microcopes**= = = Synchotron ||  || Gives an erect 3-D view of the specimen. Specimen size measured in millimetres. || //**Digital microscope**// Various types that allow the capture of digital images. || //**Compund or light microscope**// Has more than one lens and its own source of light. Can be monocular or binocular. Specimen size can be measured in micrometres. || //**Electron microscope**// Uses beams of electrons (not light) to capture very small specimens (in nanometres) || Other Types || At St Columba's we have the dyno light which is more like the stereo microscope.but it allows you to capture digital omages of your pecimen for publishing. || **Uses:** Used to measure minute particles such as viruses and bacteria. As well as eukaryotic cells,transparent thin tissues and other specimens. || **Uses:** Used to take very detailed images such as surfaces and hair tyoes of insect or cell surfaces.... ||  || =**Practice Prac. - Using a microscope**= to ensure you use microscope correctly.
 * [[image:stereo microscope.jpg]] || [[image:dyno digital microscope.png]] || [[image:light microscope.jpg width="165" height="155"]] || [[image:electron microscope.png]] || X-rays
 * //**Stereo or dissecting Microscope**//
 * You can view large and solid surfaces or specimens. Used in microsurgery. || **Uses:**
 * Following document explains procedures on how to use an electron microscope.**
 * Activit****y 1- What do you see under Light microscope*** Read handout Using a Compound Microscope
 * Use light microscope (lowest magnification) to observe an “e” from a newspaper. As the e is quite big probably the lowest magnification is suitable for this activity. The "e" should be placed on slide in the write direction i.e. facing you.
 * What do you see under the microscope? Draw the "e" How is the "e" positioned?
 * Use a ruler to estimate the size of the letter "e". What does this tell you about the field of view of the magnification you used?


 * Activit****y 2 - Drawing specimens under the microscope**
 * Read Biozone -Biological Drawings. In your groups discuss the rules about drawing specimens under microscope.
 * Use light microscope to draw a prepared microscope slide. Choose a red or white blood cell and onion cell if available.
 * Make accurate drawings at smallest and highest magnification. Identify magnification of drawings. Include the field of view in your drawings and draw the cells relative to this field of view.
 * Activity 3 - Pond Water**
 * Collect a drop of pond water and place it on a slide with a groove in it (if available).
 * Add a cover slip over the drop. It is best to wet one side of coverslip before you use forceps to lower it down.
 * Look under microscope and draw different cells that you can see.


 * Activity 4 - Creating a wet mount**
 * Watch the video below on how to prepare a slide using a wet mount.
 * **//Making a wet mount//** || media type="youtube" key="qSsMe_OXv-0" height="315" width="560" ||
 * Prepare a wet mount using onion skin cells. Draw the cells you see under the microscope. Can you see the following structure- Cell wall, cell membrane, nucleus or vacuoles.
 * Repeat using Iodine. Draw what you see. How is it different from first slide?
 * Repeat using saline solution and iodine. Draw what you see. How is it different?
 * Write a short paragraph explaining what you have learnt.
 * Write a short paragraph explaining what you have learnt.


 * Cell Structure and Function - Lecture**


 * - Field of View and scales**
 * Add a scale to your biological drawings. Before you do this we need to learn how to determine the Field of View and use this to create a scale.

=Field of View=

The field of view of a microscope refers to the circular area seen when looking down a microscope. For the particular microscope in the biology lab, the diameter of the field of view for each magnification is given below. // Note 1000um = 1 mm //
 * **Magnification** || **Diameter of field of view** ||
 * X 1000 || 180 um ||
 * X 400 || 450 um ||
 * X 100 || 1800 um ||
 * x 40 || 4500 um ||
 * Estimating Size of cells - drawing a scale**
 * //Field of view//** [[file:Field of View.pdf]]


 * Using scales to measure size**
 * Cell size Uses scale to measure cell si || media type="custom" key="25075254" ||


 * The following will help you better understand how to use the microscope to measure scale size.**
 * Scale of the Small gives you some idea about scales and measurements at very small scales. Gives you an over all picture of how small is small || media type="custom" key="25075210" ||
 * Making measurements under the microscope This is a good video that explains how to work out the size of objects seen under microscope once you know the field of view || media type="custom" key="25075222" ||
 * Field of view This video is a little slow but does explain how to work out the size of the field of view at low power and high power || media type="custom" key="25075226" ||
 * Measuring objects, scaling drawings ||  ||
 * Measuring objects, scaling drawings ||  ||

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Resources

Different types of Microcopes Best Microscope reviews Different types of Microscopes Australian Synchotron